KMID : 0364820220580040245
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Korean Journal of Microbiology 2022 Volume.58 No. 4 p.245 ~ p.254
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Molecular genetic analysis of Actinobacterial odorous substances (geosmin, 2-MIB) in North Han River watershed
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Soung Seung-Hyeok
Kim Keon-Hee Hwang Soon-jin Oh Young-Taek Park Seok-Hyo Lee Soo-Gon Jeong Hye-In Han Song-Ih
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Abstract
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This study examined the distribution of Actinobacterial odorous substances (geosmin, 2-methylisoborneol), emitting moldy and earthy odors from water and sediment in the North-Han River watershed. The potential and expression levels of the Actinobacterial geosmin and 2-MIB synthase gene were confirmed using an Actinobacteria-specific primer. The distribution of the Actinobacteria was confirmed to be 1.04?1.79 ¡¿ 104 copies/ml in water and 2.9 ¡¿ 103?4 ¡¿ 1015 copies/g in sediment of the North-Han River watershed. The distribution of the odors synthase gene by Actinobacteria was confirmed to be 1.05?3.98 ¡¿ 1013 copies/ml(g), the odors synthase expression level in Actinobacteria was confirmed to be 0.21?2.67 ¡¿ 108 copies/ml(g), and the gene expression level was found to vary in proportion to the gene potential. Furthermore, the odors synthase genes in sediment were 102?1014 times higher than in water.
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KEYWORD
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Actinobacteria, 2-MIB, geosmin, North Han River, odor
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